Lexogen, a leader in RNA sequencing technologies, is thrilled to announce the launch of the RiboCop rRNA Depletion Kit for Fish, a dedicated solution to facilitate transcriptome analysis of fish species by effectively removing unwanted ribosomal RNA (rRNA) from total RNA samples. Unobstructed Transcriptome Analysis for Fish Zebrafish ( Danio rerio ) is an important model organism for developmental biology and is also used as human disease model for drug screening and biomarker discovery. Other fish species are important indicators used for environmental surveillance or used for food production in aquacultures.
Transcriptome analysis of various fish species provides insight into developmental and regulatory processes, allows to monitor the quality of environmental conditions or detect infections with pathogens and immune responses. Total RNA from fish is predominantly composed of ribosomal RNA (rRNA), accounting for ~86 % of all transcripts. The RiboCop rRNA Depletion Kit for Fish effectively removes undesired cytoplasmic (5.
8S, 18S, and 28S) and mitochondrial (mt16S, mt12S, and 5S) rRNA, providing a comprehensive view of the fish transcriptome. Ribodepletion allows analysis of coding mRNAs as well as non-coding and non-polyadenylated transcripts for deeper insights into fish transcriptomes and expression networks covering a variety of RNA subclasses. Seamless Integration with Next-Generation Sequencing RiboCop uses a set of affinity probes and an enzyme-free workflow which pr.